Agilent bioanalyzer rna nano manual

Assess the size and quality of the input RNA by running the RNA sample on an Agilent Bioanalyzer RNA Nano/Pico Chip to determine the RNA Integrity Number (RIN). In the RNA-Seq library preparation application, RNA with different RIN values require different fragmentation times or no fragmentation at all. RNA Sample Requirements: The RNA.  · The Agilent Bioanalyzer is marked with this symbol when the user should refer to the Site Preparation and Safety Manual in order to protect the Agilent Bioanalyzer against damage. The Bioanalyzer Small RNA assay provides a fast and sensitive analysis to resolve small nucleic acid samples in the size range of 6 to nt.  · 1 Protocol for Bioanalyzer RNA nano chip. Preparation of material. Cleaning, gel preparation (start 40min before experiment) Prepare chip for measurement. Start the run. Wash, export data. Expected results. Test run with mouse total RNA. 2 Protocol for Bioanalyzer RNA pico chip.

Preparing the Agilent Bioanalyzer Instrument Preparing the Agilent Bioanalyzer Instrument 1 Make sure a clean electrode cartridge is installed. 2 If you want to change the cartridge, follow the instructions in “Loading the Electrophoresis Chip into the Bioanalyzer Instrument” on page The Agilent Bioanalyzer is marked with this symbol when the user should refer to the Site Preparation and Safety Manual in order to protect the Agilent Bioanalyzer against damage. The Bioanalyzer Small RNA assay provides a fast and sensitive analysis to resolve small nucleic acid samples in the size range of 6 to nt. G Agilent Bioanalyzer Installation and Safety Manual G Agilent Bioanalyzer Maintenance and Troubleshooting Guide Publication Number Title G Agilent Bioanalyzer – How to Use Multimedia CD-ROM Publication Number Title G Kit Guide Binder English (including all Reagent Kit Guides).

Checking RNA quality with the BIOANALYZER using the RNA nano kit. Principle. Agilent RNA kits contain chips and reagents designed for analysis of RNA. All chips were run on the Agilent Bioanalyzer using the Eukaryotic Total RNA Nano Assay software protocol. The concentration and RIN of. 9 Mei It is produced by Agilent and widely used, among other things, in RNA quality control 1 Protocol for Bioanalyzer RNA nano chip.